Role of adenomatous polyposis coli in proliferation and differentiation of colon epithelial cells in organoid culture.

ABSTRACT: Adenomatous polyposis coli (APC) is a tumor-suppressing protein whose inactivation triggers the formation of colorectal polyps. Numerous studies using cell lines or genetically engineered mice have revealed its role in suppressing Wnt/β-catenin signaling pathway and regulating cell proliferation and differentiation. Here, we performed genetic analyses of APC using a three-dimensional organoid culture of mouse colon epithelia, which enables the detailed examination of epithelial properties. Analyses of Apc-knockout colon organoids not only confirmed the importance of APC in suppressing Wnt/β-catenin signaling and regulating cell differentiation, but also revealed several novel features: a significant decrease in proliferating speed and an increase in cross-sectional area of cells. Moreover,

SUBMITTER: Yamazaki D 

PROVIDER: S-EPMC7889860 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature