A multiwell plate-based system for toxicity screening under multiple static or cycling oxygen environments.

ABSTRACT: Tumor tissue contains a continuous distribution of static and dynamically changing oxygen environments with levels ranging from physiologically normal oxygen down to anoxia. However, in vitro studies are often performed under oxygen levels that are far higher than those found in vivo. A number of devices are available to alter the oxygen environment in cell culture, including designs from our laboratory. However, in our devices and most other designs, changing the media in order to feed or dose cells remains a disruptive factor in maintaining a consistent hypoxic environment. This report presents a novel 96-well plate design that recirculates the local oxygen environment to shield cells during media changes and facilitates toxicity studies of cells cultured under varying oxygen levels. The

SUBMITTER: Yao M 

PROVIDER: S-EPMC7890056 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature