Functional assays to assess the therapeutic potential of extracellular vesicles.

ABSTRACT: An important aspect in the development of extracellular vesicle (EV) therapeutics is identifying and quantifying the key features defining their identity, purity, sterility, potency and stability to ensure batch-to-batch reproducibility of their therapeutic efficacy. Apart from EV-inherent features, therapeutic efficacy depends on a variety of additional parameters, like dosing, frequency of application, and administration route, some of which can be addressed only in clinical trials. Before initiating clinical trials, EV-inherent features should be tested in well-standardized quantitative assays in vitro or in appropriate animal models in vivo. Ideally, such assays would predict if a particular EV preparation has the potential to achieve its intended therapeutic effects, and

SUBMITTER: Nguyen VVT 

PROVIDER: S-EPMC7890556 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature