CERT_L reduces C16 ceramide, amyloid-β levels, and inflammation in a model of Alzheimer's disease.

ABSTRACT:

Background

Dysregulation of ceramide and sphingomyelin levels have been suggested to contribute to the pathogenesis of Alzheimer's disease (AD). Ceramide transfer proteins (CERTs) are ceramide carriers which are crucial for ceramide and sphingomyelin balance in cells. Extracellular forms of CERTs co-localize with amyloid-β (Aβ) plaques in AD brains. To date, the significance of these observations for the pathophysiology of AD remains uncertain.

Methods

A plasmid expressing CERT_L, the long isoform of CERTs, was used to study the interaction of CERT_L with amyloid precursor protein (APP) by co-immunoprecipitation and immunofluorescence in HEK cells. The recombinant CERT_L protein was employed to study interaction of CERT_L with amyloid-

SUBMITTER: Crivelli SM 

PROVIDER: S-EPMC7890977 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature