Dynamics of myogenic differentiation using a novel Myogenin knock-in reporter mouse.

ABSTRACT:

Background

Myogenin is a transcription factor that is expressed during terminal myoblast differentiation in embryonic development and adult muscle regeneration. Investigation of this cell state transition has been hampered by the lack of a sensitive reporter to dynamically track cells during differentiation.

Results

Here, we report a knock-in mouse line expressing the tdTOMATO fluorescent protein from the endogenous Myogenin locus. Expression of tdTOMATO in Myog^ntdTom mice recapitulated endogenous Myogenin expression during embryonic muscle formation and adult regeneration and enabled the isolation of the MYOGENIN⁺ cell population. We also show that tdTOMATO fluorescence allows tracking of differentiating myoblasts in vitro and by intravital imaging in

SUBMITTER: Benavente-Diaz M 

PROVIDER: S-EPMC7890983 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature