Differential mitochondrial proteomic analysis of A549 cells infected with avian influenza virus subtypes H5 and H9.

ABSTRACT:

Background

Both the highly pathogenic avian influenza (HPAI) H5N1 and low pathogenic avian influenza (LPAI) H9N2 viruses have been reported to cross species barriers to infect humans. H5N1 viruses can cause severe damage and are associated with a high mortality rate, but H9N2 viruses do not cause such outcomes. Our purpose was to use proteomics technology to study the differential expression of mitochondrial-related proteins related to H5N1 and H9N2 virus infections.

Methods

According to the determined viral infection titer, A549 cells were infected with 1 multiplicity of infection virus, and the mitochondria were extracted after 24 h of incubation. The protein from lysed mitochondria was analyzed by the BCA method to determine the protein concentration, as well as SDS-PAGE

SUBMITTER: Yang Y 

PROVIDER: S-EPMC7891018 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature