Intestinal crypt-derived enteroid coculture in presence of peristaltic longitudinal muscle myenteric plexus.

ABSTRACT: The role of enteric neurons in driving intestinal peristalsis has been known for over a century. However, in recent decades, scientists have begun to unravel additional complex interactions between this nerve plexus and other cell populations in the intestine. Investigations into these potential interactions are complicated by a paucity of tractable models of these cellular relationships. Here, we describe a novel technique for ex vivo coculture of enteroids, so called "mini-guts," in juxtaposition to the longitudinal muscle myenteric plexus (LMMP). Key to this system, we developed a LMMP culture media that: (i) allows the LMMP to maintain ex vivo peristalsis for 2 weeks along with proliferation of neurons, glia, smooth muscle and fibroblast cells, and (ii) supports the proli

SUBMITTER: Levin DE 

PROVIDER: S-EPMC7891127 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature