Construction and comprehensive characterization of an EcLDCc-CatIB set-varying linkers and aggregation inducing tags.

ABSTRACT:

Background

In recent years, the production of inclusion bodies that retained substantial catalytic activity was demonstrated. These catalytically active inclusion bodies (CatIBs) were formed by genetic fusion of an aggregation inducing tag to a gene of interest via short linker polypeptides and overproduction of the resulting gene fusion in Escherichia coli. The resulting CatIBs are known for their high stability, easy and cost efficient production, and recyclability and thus provide an interesting alternative to conventionally immobilized enzymes.

Results

Here, we present the construction and characterization of a CatIB set of the lysine decarboxylase from Escherichia coli (EcLDCc), constructed via Golden Gate Assembly. A total of ten EcLDCc variants consisting of combinati

SUBMITTER: Kusters K 

PROVIDER: S-EPMC7891155 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature