A novel method to identify Post-Aire stages of medullary thymic epithelial cell differentiation.

ABSTRACT: Autoimmune regulator⁺ (Aire) medullary thymic epithelial cells (mTECs) play a critical role in tolerance induction. Several studies demonstrated that Aire⁺ mTECs differentiate further into Post-Aire cells. Yet, the identification of terminal stages of mTEC maturation depends on unique fate-mapping mouse models. Herein, we resolve this limitation by segmenting the mTEC^hi (MHCII^hi CD80^hi ) compartment into mTEC^A/hi (CD24^- Sca1^- ), mTEC^B/hi (CD24⁺ Sca1^- ), and mTEC^C/hi (CD24⁺ Sca1⁺ ). While mTEC^A/hi included mostly Aire-expressing cells, mTEC^B/hi contained Aire⁺ and Aire^- cells and mTEC^C/hi

SUBMITTER: Ferreirinha P 

PROVIDER: S-EPMC7891440 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature