Transient Support from Fibroblasts is Sufficient to Drive Functional Vascularization in Engineered Tissues.

ABSTRACT: Formation of capillary blood vasculature is a critical requirement for native as well as engineered organs and can be induced in vitro by co-culturing endothelial cells with fibroblasts. However, whether these fibroblasts are required only in the initial morphogenesis of endothelial cells or needed throughout is unknown, and the ability to remove these stromal cells after assembly could be useful for clinical translation. In this study, we introduce a technique termed CAMEO (Controlled Apoptosis in Multicellular Tissues for Engineered Organogenesis), whereby fibroblasts are selectively ablated on demand, and utilize it to probe the dispensability of fibroblasts in vascular morphogenesis. The presence of fibroblasts is shown to be necessary only during the first few days of endothelial cell

SUBMITTER: Song HG 

PROVIDER: S-EPMC7891457 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature