Identification of a stool long non-coding RNAs panel as a potential biomarker for early detection of colorectal cancer.

ABSTRACT:

Background

The feces of colorectal cancer (CRC) patients contain tumor colonocytes, which constantly shed into the lumen area. Therefore, stool evaluation can be considered as a rapid and low-risk way to directly determine the colon and rectum status. As long non-coding RNAs (lncRNAs) alterations are important in cancer cells fate regulation, we aimed to assess the level of a panel of cancer-related lncRNAs in fecal colonocytes.

Methods

The population study consisted of 150 subjects, including a training set, a validation set, and a group of 30 colon polyps. The expression levels of lncRNAs were evaluated by quantitative real-time PCR (qRT-PCR). The NPInetr and EnrichR tools were used to identify the interactions and functions of lncRNAs.

Results

A total of 10 signifi

SUBMITTER: Gharib E 

PROVIDER: S-EPMC7891513 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature