Mass Cytometry Defines Virus-Specific CD4⁺ T Cells in Influenza Vaccination.

ABSTRACT: The antiviral response to influenza virus is complex and multifaceted, involving many immune cell subsets. There is an urgent need to understand the role of CD4⁺ T cells, which orchestrate an effective antiviral response, to improve vaccine design strategies. In this study, we analyzed PBMCs from human participants immunized with influenza vaccine, using high-dimensional single-cell proteomic immune profiling by mass cytometry. Data were analyzed using a novel clustering algorithm, denoised ragged pruning, to define possible influenza virus-specific clusters of CD4⁺ T cells. Denoised ragged pruning identified six clusters of cells. Among these, one cluster (Cluster 3) was found to increase in abundance following stimulation with influenza virus peptide ex vivo. A sepa

SUBMITTER: Subrahmanyam PB 

PROVIDER: S-EPMC7891553 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature