Dissecting the roles of Cse1 and Nup2 in classical NLS-cargo release in vivo.

ABSTRACT: The importin α/β transport machinery mediates the nuclear import of cargo proteins that bear a classical nuclear localization sequence (cNLS). These cargo proteins are linked to the major nuclear protein import factor, importin-β, by the importin-α adapter, after which cargo/carrier complexes enter the nucleus through nuclear pores. In the nucleus, cargo is released by the action of RanGTP and the nuclear pore protein Nup2, after which the importins are recycled to the cytoplasm for further transport cycles. The nuclear export of importin-α is mediated by Cse1/CAS. Here, we exploit structures of functionally important complexes to identify residues that are critical for these interactions and provide insight into how cycles of protein import and recycling of importin-α occur in vivo using

SUBMITTER: Lange A 

PROVIDER: S-EPMC7891619 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature