A Comparative Analysis of Reactive Muller Glia Gene Expression After Light Damage and microRNA-Depleted Muller Glia-Focus on microRNAs.

ABSTRACT: Müller glia (MG) are the predominant glia in the neural retina and become reactive after injury or in disease. microRNAs (miRNAs) are translational repressors that regulate a variety of processes during development and are required for MG function. However, no data is available about the MG miRNAs in reactive gliosis. Therefore, in this study, we aimed to profile miRNAs and mRNAs in reactive MG 7 days after light damage. Light damage was performed for 8 h at 10,000 lux; this leads to rapid neuronal loss and strong MG reactivity. miRNAs were profiled using the Nanostring platform, gene expression analysis was conducted via microarray. We compared the light damage dataset with the dataset of Dicer deleted MG in order to find similarities and differences. We found: (1) The vast majority of MG

SUBMITTER: Kang S 

PROVIDER: S-EPMC7891663 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature