Characterization of the G protein-coupled receptor kinase 6 promoter reveals a functional CREB binding site.

ABSTRACT:

Background

G protein-coupled receptor kinase 6 (GRK6) is part of the G protein-coupled receptor kinase family, whose members act as key regulators of seven-transmembrane receptor signalling. GRK6 seems to play a role in regulation of inflammatory processes, but mechanisms of transcriptional regulation of GRK6 expression in inflammatory cell lines have not been characterized. Protein kinase C (PKC) signalling is also involved in inflammatory regulation and an impact of PKC activation on GRK6 protein expression was described previously. Thus, the aim of this study was to 1) characterize the GRK6 promoter, and 2) investigate a potential influence of PKC on GRK6 expression.

Methods

Five deletion constructs of the GRK6 promoter were cloned. After transient transfection into a hum

SUBMITTER: Stegen M 

PROVIDER: S-EPMC7891717 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature