Quantitative analysis of seven plant hormones in Lotus japonicus using standard addition method.

ABSTRACT: Plant hormones have been identified to be versatile signaling molecules essential for plant growth, development, and stress response. Their content levels vary depending on the species, and they also change in response to any external stimuli. Thus, simultaneous quantification of multiple plant hormones is required to understand plant physiology. Sensitive and quantitative analysis using liquid chromatography-linked mass spectrometry (LC-MS/MS) has been used in detecting plant hormones; however, quantification without stable isotopes is yet to be established. In this study, we quantified seven representative plant hormones of Lotus japonicus, which is a model legume for standard addition method. Accurate masses for monoisotopic ions of seven phytohormones were determined for high-resolutio

SUBMITTER: Hashiguchi T 

PROVIDER: S-EPMC7891737 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature