CircUSP36 knockdown alleviates oxidized low‑density lipoprotein‑induced cell injury and inflammatory responses in human umbilical vein endothelial cells via the miR‑20a‑5p/ROCK2 axis.

ABSTRACT: The dysfunctions of human umbilical vein endothelial cells (HUVECs) are important features of atherosclerosis (AS). Circular RNAs (circRNAs) are regulators of a wide range of human diseases, including AS. The present study aimed to investigate the role of circUSP36 in the ectopic phenotype of HUVECs and to provide evidence of the involvement of circUSP36 in the pathogenesis of AS. AS cell models in vitro were established using HUVECs exposed to oxidized low‑density lipoprotein (ox‑LDL). Cell viability, cell cycle progression and apoptosis, and cell migration and invasion were assessed by cell counting kit‑8 (CCK‑8) assay, flow cytometric assay and Transwell assay, respectively. The expression levels or releases of pro‑inflammatory factors were detected by western blot analysis or en

SUBMITTER: Miao J 

PROVIDER: S-EPMC7891832 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature