Prognostic Utility of Whole-Genome Sequencing and Polymerase Chain Reaction Tests of Ocular Fluids in Postprocedural Endophthalmitis.

ABSTRACT:

Purpose

To associate detection of potential pathogen DNA in endophthalmitis with clinical outcomes.

Design

Prospective cohort study.

Methods

Patients in whom endophthalmitis was diagnosed following an intraocular procedure were recruited. Clinical outcome data from baseline, week-1, month-1, and month-3 visits were collected. Intraocular biopsy samples were cultured by standard methods. Quantitative polymerase chain reaction (qPCR) was performed for specific pathogens and whole-genome sequencing (WGS).

Results

A total of 50 patients (mean age 72 years old; 52% male) were enrolled. Twenty-four cases were culture-positive and 26 were culture-negative. WGS identified the cultured organism in 76% of culture-positive cases and identified potential pathogens in 33% o

SUBMITTER: Lee CS 

PROVIDER: S-EPMC7891843 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature