ClC-5 Downregulation Induces Osteosarcoma Cell Apoptosis by Promoting Bax and tBid Complex Formation.

ABSTRACT: Osteosarcoma is the most common malignant bone tumor. Chloride (Cl^-) channels-mediated Cl^- movement plays an important role in regulating the functions of various cancer cells, but its role in osteosarcoma remains unclear. In this study, we found that ClC-5 was increased in osteosarcoma tissues compared with normal bone tissues. Patients with high ClC-5 expression showed poor overall survival relative to those patients with low ClC-5 expression. Higher ClC-5 expression and lower intracellular Cl^- concentration ([Cl^-]_i) were observed in osteosarcoma cells compared with normal osteoblasts. Lowering [Cl^-]_i increased the viability of osteosarcoma cells, which was markedly blocked by ClC-5 downregulation. Knockdown of

SUBMITTER: Peng F 

PROVIDER: S-EPMC7892965 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature