Affinity-based proteomics reveals novel binding partners for Rab46 in endothelial cells.

ABSTRACT: Rab46 is a novel Ca²⁺-sensing Rab GTPase shown to have important functions in endothelial and immune cells. The presence of functional Ca²⁺-binding, coiled-coil and Rab domains suggest that Rab46 will be important for coupling rapid responses to signalling in many cell types. The molecular mechanisms underlying Rab46 function are currently unknown. Here we provide the first resource for studying Rab46 interacting proteins. Using liquid chromatography tandem mass spectrometry (LC-MS/MS) to identify affinity purified proteins that bind to constitutively active GFP-Rab46 or inactive GFP-Rab46 expressed in endothelial cells, we have revealed 922 peptides that interact with either the GTP-bound Rab46 or GDP-bound Rab46. To identify proteins that could be potential Rab46 ef

SUBMITTER: Pedicini L 

PROVIDER: S-EPMC7893075 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature