Genetic Diversity of Astrovirus in Children From a Birth Cohort in Nepal.

ABSTRACT: Objective: This study describes the types of Human astroviruses detected in stool samples collected from a birth cohort of children in Nepal. Methods: Using a commercial kit (ProSpecT), a total of 5,224 diarrheal and non-diarrheal stool samples were screened for Human astrovirus by ELISA. RT-PCR was performed on ELISA positive samples (2.8%) for further confirmation. The primary RT-PCR assay used targets the ORF2 region and detects human astrovirus type 1-8. Samples that were negative in this assay were further analyzed using primers that target the ORF1b region of human astrovirus which detect both classical type (HAstV 1-8) and novel types (MLB1-5, VA 1-5). PCR positive samples were analyzed by Sanger sequencing to determine the genotype. Results: A total of 148 avai

SUBMITTER: Shrestha SK 

PROVIDER: S-EPMC7893100 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature