Evaluating Accuracy of DNA Pool Construction Based on White Blood Cell Counts.

ABSTRACT: Pooling individual samples prior to DNA extraction can mitigate the cost of DNA extraction and genotyping; however, these methods need to accurately generate equal representation of individuals within pools. The objective of this study was to determine accuracy of pool construction of blood samples based on white blood cell counts compared to two common DNA quantification methods. Fifty individual bovine blood samples were collected, and then pooled with all individuals represented in each pool. Pools were constructed with the target of equal representation of each individual animal based on number of white blood cells, spectrophotometric readings, spectrofluorometric readings, and whole blood volume with 9 pools per method and a total of 36 pools. Pools and individual samples that compris

SUBMITTER: Abrams AN 

PROVIDER: S-EPMC7893106 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature