Genome mining combined metabolic shunting and OSMAC strategy of an endophytic fungus leads to the production of diverse natural products.

ABSTRACT: Endophytic fungi are promising producers of bioactive small molecules. Bioinformatic analysis of the genome of an endophytic fungus Penicillium dangeardii revealed 43 biosynthetic gene clusters, exhibited its strong ability to produce numbers of secondary metabolites. However, this strain mainly produce rubratoxins alone with high yield in varied culture conditions, suggested most gene clusters are silent. Efforts for mining the cryptic gene clusters in P. dangeardii, including epigenetic regulation and one-strain-many-compounds (OSMAC) approach were failed probably due to the high yield of rubratoxins. A metabolic shunting strategy by deleting the key gene for rubratoxins biosynthesis combining with optimization of culture condition successfully activated multiple silent gen

SUBMITTER: Wei Q 

PROVIDER: S-EPMC7893140 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature