Sonic Hedgehog receptor Patched deficiency in astrocytes enhances glucose metabolism in mice.

ABSTRACT:

Objective

Astrocytes are glial cells proposed as the main Sonic hedgehog (Shh)-responsive cells in the adult brain. Their roles in mediating Shh functions are still poorly understood. In the hypothalamus, astrocytes support neuronal circuits implicated in the regulation of energy metabolism. In this study, we investigated the impact of genetic activation of Shh signaling on hypothalamic astrocytes and characterized its effects on energy metabolism.

Methods

We analyzed the distribution of gene transcripts of the Shh pathway (Ptc, Gli1, Gli2, and Gli3) in astrocytes using single molecule fluorescence in situ hybridization combined with immunohistofluorescence of Shh peptides by Western blotting in the adult mouse hypothalamus. Based on the metabolic phenotype, we characterized

SUBMITTER: Tirou L 

PROVIDER: S-EPMC7893488 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature