Mode of Action of Disinfection Chemicals on the Bacterial Spore Structure and Their Raman Spectra.

ABSTRACT: Contamination of toxic spore-forming bacteria is problematic since spores can survive a plethora of disinfection chemicals and it is hard to rapidly detect if the disinfection chemical has inactivated the spores. Thus, robust decontamination strategies and reliable detection methods to identify dead from viable spores are critical. In this work, we investigate the chemical changes of Bacillus thuringiensis spores treated with sporicidal agents such as chlorine dioxide, peracetic acid, and sodium hypochlorite using laser tweezers Raman spectroscopy. We also image treated spores using SEM and TEM to verify if we can correlate structural changes in the spores with changes to their Raman spectra. We found that over 30 min, chlorine dioxide did not change the Raman spectrum or the spore

SUBMITTER: Malyshev D 

PROVIDER: S-EPMC7893628 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature