Discovery and validation of FBLN1 and ANT3 as potential biomarkers for early detection of cervical cancer.

ABSTRACT:

Background

To validate markers for cervical carcinoma (CC) and precancerous lesions related with HPV infections.

Methods

Three different cervical cancer cell lines C-33A, SiHa and Caski were used for secretome profiling by label-free quantitative proteomics. Cervical exfoliated cells and matching serum samples were collected from 284 patients with normal control (n = 75, 26.41 %), precancerous lesions (n = 88, 30.99 %) and early stage cervical squamous carcinoma (n = 121, 42.61 %). HPV subtyping and quantification was performed by PCR and hybridization. 20 candidate proteins identified in previous screening studies (tissue, plasma, cells) were quantified by ELISA. Finally, highly quantitative parallel reaction monitoring mass spectrometry was used to assess the specificities

SUBMITTER: Hao Y 

PROVIDER: S-EPMC7893763 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature