Assessing optimal: inequalities in codon optimization algorithms.

ABSTRACT:

Background

Custom genes have become a common resource in recombinant biology over the last 20 years due to the plummeting cost of DNA synthesis. These genes are often "optimized" to non-native sequences for overexpression in a non-native host by substituting synonymous codons within the coding DNA sequence (CDS). A handful of studies have compared native and optimized CDSs, reporting different levels of soluble product due to the accumulation of misfolded aggregates, variable activity of enzymes, and (at least one report of) a change in substrate specificity. No study, to the best of our knowledge, has performed a practical comparison of CDSs generated from different codon optimization algorithms or reported the corresponding protein yields.

Results

In our efforts to underst

SUBMITTER: Ranaghan MJ 

PROVIDER: S-EPMC7893858 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature