Global transcriptome profile of the developmental principles of in vitro iPSC-to-motor neuron differentiation.

ABSTRACT:

Background

Human induced pluripotent stem cells (iPSC) have opened new avenues for regenerative medicine. Consequently, iPSC-derived motor neurons have emerged as potentially viable therapies for spinal cord injuries and neurodegenerative disorders including Amyotrophic Lateral Sclerosis. However, direct clinical application of iPSC bears in itself the risk of tumorigenesis and other unforeseeable genetic or epigenetic abnormalities.

Results

Employing RNA-seq technology, we identified and characterized gene regulatory networks triggered by in vitro chemical reprogramming of iPSC into cells with the molecular features of motor neurons (MNs) whose function in vivo is to innervate effector organs. We present meta-transcriptome signatures of 5 cell types: iPSCs, neural stem cell

SUBMITTER: Solomon E 

PROVIDER: S-EPMC7893891 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature