In situ measurements of oxidation-reduction potential and hydrogen peroxide concentration as tools for revealing LPMO inactivation during enzymatic saccharification of cellulose.

ABSTRACT:

Background

Biochemical conversion of lignocellulosic biomass to simple sugars at commercial scale is hampered by the high cost of saccharifying enzymes. Lytic polysaccharide monooxygenases (LPMOs) may hold the key to overcome economic barriers. Recent studies have shown that controlled activation of LPMOs by a continuous H₂O₂ supply can boost saccharification yields, while overdosing H₂O₂ may lead to enzyme inactivation and reduce overall sugar yields. While following LPMO action by ex situ analysis of LPMO products confirms enzyme inactivation, currently no preventive measures are available to intervene before complete inactivation.

Results

Here, we carried out enzymatic saccharification of the model cellulose Avicel with an LPMO-

SUBMITTER: Kadic A 

PROVIDER: S-EPMC7893893 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature