Microtiter plate test using liquid medium is an alternative method for monitoring metyltetraprole sensitivity in Cercospora beticola.

ABSTRACT:

Background

Metyltetraprole is a new quinone outside inhibitor (QoI) fungicide showing potent activity against QoI-resistant fungi that possess the G143A cytochrome b mutation, which confers resistance to existing QoIs such as trifloxystrobin. For its sustainable use, monitoring of metyltetraprole sensitivity is necessary and the establishment of appropriate methodology is important in each pathogen species.

Results

In Cercospora beticola, the causal agent of sugar beet leaf spot, some isolates were less sensitive to metyltetraprole (EC₅₀  > 1 mg L^-1 , higher than the saturated concentration) using the common agar plate method, even with 100 mg L^-1 salicylhydroxamic acid, an alternative oxidase inhibitor. However, microtiter tests (EC_50</

SUBMITTER: Matsuzaki Y 

PROVIDER: S-EPMC7894156 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature