System-level analyses of keystone genes required for mammalian tooth development.

ABSTRACT: When a null mutation of a gene causes a complete developmental arrest, the gene is typically considered essential for life. Yet, in most cases, null mutations have more subtle effects on the phenotype. Here we used the phenotypic severity of mutations as a tool to examine system-level dynamics of gene expression. We classify genes required for the normal development of the mouse molar into different categories that range from essential to subtle modification of the phenotype. Collectively, we call these the developmental keystone genes. Transcriptome profiling using microarray and RNAseq analyses of patterning stage mouse molars show highly elevated expression levels for genes essential for the progression of tooth development, a result reminiscent of essential genes in single-cell organis

SUBMITTER: Hallikas O 

PROVIDER: S-EPMC7894285 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature