Effects of the histone acetylase inhibitor C646 on growth and differentiation of adipose-derived stem cells.

ABSTRACT: As an important histone acetylase, the transcriptional coactivator P300/CBP affects target gene expression and plays a role in the maintenance of stem cell characteristics and differentiation potential. In this study, we explored the action of a highly effective selective histone acetylase inhibitor, C646, on goat adipose-derived stem cells (gADSCs), and investigated the impact of histone acetylation on the growth characteristics and the differentiation potential of ADSCs. We found that C646 blocked the cell proliferation, arrested the cell cycle, and triggered apoptosis. Notably, immunocytochemistry and western blot analyses showed that the acetylation level of histone H3K9 was increased. Moreover, although real-time quantitative PCR and western blot confirmed that P300 expression was inh

SUBMITTER: Wang Z 

PROVIDER: S-EPMC7894422 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature