Comparative proteomics of Geobacter sulfurreducens PCA^T in response to acetate, formate and/or hydrogen as electron donor.

ABSTRACT: Geobacter sulfurreducens is a model bacterium to study the degradation of organic compounds coupled to the reduction of Fe(III). The response of G. sulfurreducens to the electron donors acetate, formate, hydrogen and a mixture of all three with Fe(III) citrate as electron acceptor was studied using comparative physiological and proteomic approaches. Variations in the supplied electron donors resulted in differential abundance of proteins involved in the citric acid cycle (CAC), gluconeogenesis, electron transport, and hydrogenases and formate dehydrogenase. Our results provided new insights into the electron donor metabolism of G. sulfurreducens. Remarkably, formate was the preferred electron donor compared to acetate, hydrogen, or acetate plus hydrogen. When hydrogen was the electron dono

SUBMITTER: Mollaei M 

PROVIDER: S-EPMC7894505 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature