C-Terminal Tag Location Hampers in Vitro Profiling of OGT Peptide Substrates by mRNA Display.

ABSTRACT: O-GlcNAc transferase (OGT) is the only enzyme that catalyzes the post-translational modification of proteins at Ser/Thr with a single β-N-acetylglucosamine (O-GlcNAcylation). Its activity has been associated with chronic diseases such as cancer, diabetes and neurodegenerative disease. Although numerous OGT substrates have been identified, its accepted substrate scope can still be refined. We report here an attempt to better define the peptide-recognition requirements of the OGT active site by using mRNA display, taking advantage of its extremely high throughput to assess the substrate potential of a library of all possible nonamer peptides. An antibody-based selection process is described here that is able to enrich an OGT substrate peptide from such a library, but with poor absolute recov

SUBMITTER: Shi J 

PROVIDER: S-EPMC7894566 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature