Transcriptomes of Major Proximal Tubule Cell Culture Models.

ABSTRACT:

Background

Cultured cell lines are widely used for research in the physiology, pathophysiology, toxicology, and pharmacology of the renal proximal tubule. The lines that are most appropriate for a given use depend upon the genes expressed. New tools for transcriptomic profiling using RNA sequencing (RNA-Seq) make it possible to catalog expressed genes in each cell line.

Methods

Fourteen different proximal tubule cell lines, representing six species, were grown on permeable supports under conditions specific for the respective lines. RNA-Seq followed standard procedures.

Results

Transcripts expressed in cell lines variably matched transcripts selectively expressed in native proximal tubule. Opossum kidney (OK) cells displayed the highest percentage match (45% of proxim

SUBMITTER: Khundmiri SJ 

PROVIDER: S-EPMC7894662 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature