Isolation of Natural DNA Aptamers for Challenging Small-Molecule Targets, Cannabinoids.

ABSTRACT: Aptamers are nucleic acid-based affinity reagents that are isolated via an in vitro process known as systematic evolution of ligands by exponential enrichment (SELEX). Despite their great potential for a wide range of analytical applications, there are relatively few high-quality small-molecule binding aptamers, especially for "challenging" targets that have low water solubility and/or limited moieties for aptamer recognition. The use of libraries containing chemically modified bases may improve the outcome of some SELEX experiments, but this approach is costly and yields inconsistent results. Here, we demonstrate that a thoughtfully designed SELEX procedure with natural DNA libraries can isolate aptamers with high affinity and specificity for challenging small molecules, including

SUBMITTER: Yu H 

PROVIDER: S-EPMC7895305 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature