Wide range of metabolic adaptations to the acquisition of the Calvin cycle revealed by comparison of microbial genomes.

ABSTRACT: Knowledge of the genetic basis for autotrophic metabolism is valuable since it relates to both the emergence of life and to the metabolic engineering challenge of incorporating CO2 as a potential substrate for biorefining. The most common CO2 fixation pathway is the Calvin cycle, which utilizes Rubisco and phosphoribulokinase enzymes. We searched thousands of microbial genomes and found that 6.0% contained the Calvin cycle. We then contrasted the genomes of Calvin cycle-positive, non-cyanobacterial microbes and their closest relatives by enrichment analysis, ancestral character estimation, and random forest machine learning, to explore genetic adaptations associated with acquisition of the Calvin cycle. The Calvin cycle overlaps with the pentose phosphate pathway and glycolysis, and we cou

SUBMITTER: Asplund-Samuelsson J 

PROVIDER: S-EPMC7895386 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature