The porcine corneal surface bacterial microbiome: A distinctive niche within the ocular surface.

ABSTRACT:

Purpose

The ocular surface microbiome has been described as paucibacterial. Until now, studies investigating the bacterial community associated with the ocular surface through high-throughput sequencing have focused on the conjunctiva. Conjunctival samples are thought to reflect and be representative of the microbiome residing on the ocular surface, including the cornea. Here, we hypothesized that the bacterial community associated with the corneal surface was different from those of the inferonasal and superotemporal conjunctival fornices, and from the tear film.

Methods

Both eyes from 15 healthy piglets were sampled using swabs (inferonasal fornix, superotemporal fornix, and corneal surface, n = 30 each) and Schirmer tear test strips (STT, n = 30). Negative sampling contro

SUBMITTER: Leis ML 

PROVIDER: S-EPMC7895408 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature