Artificial Metalloproteins with Dinuclear Iron-Hydroxido Centers.

ABSTRACT: Dinuclear iron centers with a bridging hydroxido or oxido ligand form active sites within a variety of metalloproteins. A key feature of these sites is the ability of the protein to control the structures around the Fe centers, which leads to entatic states that are essential for function. To simulate this controlled environment, artificial proteins have been engineered using biotin-streptavidin (Sav) technology in which Fe complexes from adjacent subunits can assemble to form [Fe^III-(μ-OH)-Fe^III] cores. The assembly process is promoted by the site-specific localization of the Fe complexes within a subunit through the designed mutation of a tyrosinate side chain to coordinate the Fe centers. An important outcome is that the Sav host can regulate the Fe···Fe separation

SUBMITTER: Miller KR 

PROVIDER: S-EPMC7895470 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature