A universal protocol for isolating retinal ON bipolar cells across species via fluorescence-activated cell sorting.

ABSTRACT: Inherited retinal dystrophies (IRDs) are characterized by progressive degeneration and loss of light-sensing photoreceptors. The most promising therapeutic approach for IRDs is gene supplementation therapy using viral vectors, which requires the presence of viable photoreceptors at the time of intervention. At later disease stages, photoreceptors are lost and can no longer be rescued with this approach. For these patients, conferring light-sensing abilities to the remaining interneurons of the ON circuit (i.e., ON bipolar cells) using optogenetic tools poses an alternative treatment strategy. Such treatments, however, are hampered by the lack of efficient gene delivery tools targeting ON bipolar cells, which in turn rely on the effective isolation of these cells to facilitate tool developm

SUBMITTER: Murenu E 

PROVIDER: S-EPMC7895692 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature