Identification and evaluation of antioxidant and reference genes for quantitative real-time PCR in blood of Caiman latirostris.

ABSTRACT: The quantitative real-time polymerase chain reaction (qPCR) has been one of the most promising approaches to perform rapid and accurate quantification of DNA in various biological systems. The aim of this study was to standardized the qPCR technique for the analysis of important genes involved in the main routes of antioxidant defense against reactive oxygen species (catalase: cat and superoxide dismutase: sod) and evaluate the stability of different reference genes in blood of Caiman latirostris hatchlings. The stability of the reference genes, β-actin, glyceraldehyde 3-phosphate dehydrogenase (gapdh) and ribosomal protein L8 (rpl8) was determined using the comparative ΔCt, NormFinder, geNorm, BestKeeper and RefFinder. Then, cat and sod<

SUBMITTER: Odetti LM 

PROVIDER: S-EPMC7895747 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature