Icariin promotes the repair of PC12 cells by inhibiting endoplasmic reticulum stress.

ABSTRACT:

Background

Endoplasmic reticulum stress (ERS) is one of the main mechanisms of spinal cord injury (SCI) pathology and can affect the physiological state of neurons. Icariin (ICA), the main pharmacological component of Epimedium, can relieve the symptoms of patients with SCI and has obvious protective effects on neurons through ERS.

Methods

PC12 cells were induced to differentiate into neurons by nerve growth factor and identified by flow cytometry. Cell proliferation was detected by CCK8 method, cell viability was detected by SRB assay, apoptosis was detected by flow cytometry and microstructure of ER was observed by transmission electron microscope. Western blot was used to detect the protein expression of CHOP and Grp78, and qPCR was used to detect the mRNA expression of C

SUBMITTER: Wu C 

PROVIDER: S-EPMC7896365 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature