An Immunocompetent Model of Pancreatic Cancer Resection and Recurrence.

ABSTRACT:

Background

Even after surgical resection, most patients with localized pancreatic ductal adenocarcinoma (PDAC) succumb to disease recurrence. Current animal models do not recapitulate this pattern of disease recurrence. Our goal was to develop a clinically relevant, immunocompetent model of PDAC resection to study recurrence and evaluate therapy.

Methods

Pancreatic cancer cells derived from tumors arising in KPC (LSL-Kras^G12D/+; LSL-Trp53^R172H/+; Pdx-1-Cre) mice were co-injected with stromal cells (pancreatic stellate cells) into the pancreas of immunocompetent mice to simulate the stroma-rich tumors seen in human PDAC. After allowing tumors to form, we resected these localized tumors and followed the mice for tumor recurrence. Circulating tumor cells (

SUBMITTER: Giri B 

PROVIDER: S-EPMC7896482 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature