Suppressing evolution in genetically engineered systems through repeated supplementation.

ABSTRACT: Genetically engineered organisms are prone to evolve in response to the engineering. This evolution is often undesirable and can negatively affect the purpose of the engineering. Methods that maintain the stability of engineered genomes are therefore critical to the successful design and use of genetically engineered organisms. One potential method to limit unwanted evolution is by taking advantage of the ability of gene flow to counter local adaption, a process of supplementation. Here, we investigate the feasibility of supplementation as a mechanism to offset the evolutionary degradation of a transgene in three model systems: a bioreactor, a gene drive, and a transmissible vaccine. In each model, continual introduction from a stock is used to balance mutation and selection against the tr

SUBMITTER: Layman NC 

PROVIDER: S-EPMC7896713 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature