Hydrogen Sulfide Is a Regulator of Hemoglobin Oxygen-Carrying Capacity via Controlling 2,3-BPG Production in Erythrocytes.

ABSTRACT: Hydrogen sulfide (H₂S) is naturally synthesized in a wide range of mammalian tissues. Whether H₂S is involved in the regulation of erythrocyte functions remains unknown. Using mice with a genetic deficiency in a H₂S natural synthesis enzyme cystathionine-γ-lyase (CSE) and high-throughput metabolomic profiling, we found that levels of erythrocyte 2,3-bisphosphoglycerate (2,3-BPG), an erythroid-specific metabolite negatively regulating hemoglobin- (Hb-) oxygen (O₂) binding affinity, were increased in CSE knockout (Cse ^-/-) mice under normoxia. Consistently, the 50% oxygen saturation (P50) value was increased in erythrocytes of Cse ^-/- mice. These effects were reversed by treatment with H₂S donor

SUBMITTER: Wang G 

PROVIDER: S-EPMC7896853 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature