Phylogenetic relationship and genotype variation of six Newcastle disease viruses isolated from duck in Indonesia.

ABSTRACT:

Background and aim

Newcastle disease viruses (NDVs) are frequently acquired from all ages and types of bird species. In general, ducks are considered as potential reservoirs for different genotypes of NDV and are resistant even to velogenic NDV strains. This research was conducted to genotypically and phylogenetically characterize NDV isolates collected from unvaccinated ducks from Indonesia.

Materials and methods

A total of 200 samples were collected through cloacal swabs and were inoculated in the allantoic sacs of 8-day-old specific pathogen-free eggs. Hemagglutination (HA) activity was analyzed through a HA test, and isolated viruses were characterized by reverse transcription-polymerase chain reaction targeting the complete fusion (F)-gene of NDV using three prim

SUBMITTER: Putri N 

PROVIDER: S-EPMC7896909 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature