Hepatic NFAT signaling regulates the expression of inflammatory cytokines in cholestasis.

ABSTRACT:

Background & aims

The nuclear factor of activated T-cells (NFAT) plays an important role in immune responses by regulating the expression of inflammatory genes. However, it is not known whether NFAT plays any role in the bile acid (BA)-induced hepatic inflammatory response. Thus, we aimed to examine the functional role of NFATc3 in cholestatic liver injury in mice and humans.

Methods

Gene and protein expression and cellular localization were assessed in primary hepatocyte cultures (mouse and human) and cholestatic liver tissues (murine models and patients with primary biliary cholangitis [PBC] or primary sclerosing cholangitis [PSC]) by quantitative PCR, western blot and immunohistochemistry. Specific NFAT inhibitors were used in vivo and in vitro. Gene reporter assays and C

SUBMITTER: Cai SY 

PROVIDER: S-EPMC7897288 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature