Rapid quantification of multi-cryoprotectant toxicity using an automated liquid handling method.

ABSTRACT: Cryopreservation in a vitrified state has vast potential for long-term storage of tissues and organs that may be damaged by ice formation. However, the toxicity imparted by the high concentration of cryoprotectants (CPAs) required to vitrify these specimens remains a hurdle. To address this challenge, we previously developed a mathematical approach to design less toxic CPA equilibration methods based on the minimization of a toxicity cost function. This approach was used to design improved methods for equilibration of bovine pulmonary artery endothelial cells (BPAEC) with glycerol. To fully capitalize on the toxicity cost function approach, it is critical to describe the toxicity kinetics of additional CPAs, including multi-CPA mixtures that are commonly used for vitrification. In this wor

SUBMITTER: Warner RM 

PROVIDER: S-EPMC7897298 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature