A supernumerary designer chromosome for modular in vivo pathway assembly in Saccharomyces cerevisiae.

ABSTRACT: The construction of microbial cell factories for sustainable production of chemicals and pharmaceuticals requires extensive genome engineering. Using Saccharomyces cerevisiae, this study proposes synthetic neochromosomes as orthogonal expression platforms for rewiring native cellular processes and implementing new functionalities. Capitalizing the powerful homologous recombination capability of S. cerevisiae, modular neochromosomes of 50 and 100 kb were fully assembled de novo from up to 44 transcriptional-unit-sized fragments in a single transformation. These assemblies were remarkably efficient and faithful to their in silico design. Neochromosomes made of non-coding DNA were stably replicated and segregated irrespective of their size without affecting the physiology of their host. These

SUBMITTER: Postma ED 

PROVIDER: S-EPMC7897487 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature