Patient-derived scaffolds as a model of colorectal cancer.

ABSTRACT:

Background

Colorectal cancer is the second most common cause of cancer-related death worldwide and standardized therapies often fail to treat the more aggressive and progressive types of colorectal cancer. Tumor cell heterogeneity and influence from the surrounding tumor microenvironment (TME) contribute to the complexity of the disease and large variability in clinical outcomes.

Methods

To model the heterogeneous nature of colorectal cancer, we used patient-derived scaffolds (PDS), which were obtained via decellularization of surgically resected tumor material, as a growth substrate for standardized cell lines.

Results

After confirmation of native cell absence and validation of the structural and compositional integrity of the matrix, 89 colorectal PDS were repopulat

SUBMITTER: Parkinson GT 

PROVIDER: S-EPMC7897946 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature